Fig. 1. Tec29 is expressed in embryonic salivary gland and has a salivary gland phenotype. In all the figures, anterior is towards the left. (A-D) Tec29 mRNA is expressed in the salivary placodes at stage 11 and in the invaginating salivary glands (arrowhead). Tec29 is also expressed in the salivary ducts from stage 12 onwards (D, arrow). (E,F) The shorter isoform of Tec29, type 1, is expressed in the salivary primordium (E), while the longer isoform, type 2, is expressed only in the nervous system (F). (G,H) Immunostaining for TEC29 protein shows that it is expressed in the salivary placodes and is apically localized in the invaginating gland (white arrowhead). (I-L) Immunostaining with anti-CREB antibody showed that multiple alleles of Tec29, Tec29^K00206, Tec29^K00206/Tec29^K05610 and Tec29^e482 have long salivary glands compared with w¹¹¹⁸ embryos (I). (M,N) The salivary ducts in Tec29^K00206, as visualized by dead ringer (dri), do not go through normal morphogenesis (arrows). (O,P) Double labeling for FKH and EN show that the salivary placodes at stage 11 are normal and respect their AP boundary in Tec29^K00206 embryos.