Fig. 4. Actin is disorganized in the salivary placodes of Tec29^K00206 embryos. All the panels show optical sections of embryos focused on the apical surface of the salivary placodes. In all the panels, the circle outlines the salivary placodes. (A,B) The apical staining for -spectrin looks normal in Tec29^K00206 embryos. (C,D) Actin disorganization on the apical surface of the cells in Tec29^K00206 embryos is evident at early stage 12 when the embryos are stained with a monoclonal antibody against ACTIN, which detects both G-actin and F-actin.