Fig. 5. Reduced diencephalic and myelencephalic CPe after late Gdf7-mediated ablation. Ttr in situ hybridization (A-D,I-L), fluorescent TUNEL assays (E,F,M,N; TUNEL green, nuclei blue, red blood cells yellow-orange), and Hematoxylin and Eosin (G,H,O,P; H&E) stains of E12.5-16.5 Gdf7Cre;Gdf7DTA coronal cryosections. (A-H) Diencephalon. The anterior diencephalic midline shows markedly reduced Ttr expression (A,C), increased apoptosis (E) and lack of papillary CP (G) in mutants compared with controls (B,D,F,H). (I-P) Hindbrain. As in the diencephalon, the mutant hindbrain shows reduced Ttr expression (I,K), increased apoptosis (M) and a lack of papillarity (O). Arrows designate CPe or its expected location; arrowheads designate TUNEL-positive cells; broken lines delimit the CPe. Scale bars: 0.4 mm in K,L; 0.1 mm in A-J,M-O.