Fig. 6. Preserved telencephalic CPe after late Gdf7-mediated ablation. Ttr in situ hybridization (A-D), fluorescent TUNEL assays (E-H), and Hematoxylin and Eosin staining (I,J) as in Fig. 5. Anterior sections (A,B) at level of choroid plaque; posterior sections (C,D) at level of anterior diencephalon. Gdf7Cre;Gdf7DTA mutants maintain strong Ttr expression in the E12.5 telencephalon (A,C), in contrast to the diencephalon (arrow in C). Anterior tCPe volume appears slightly reduced (A) compared with normal (B). Significant tCPe apoptosis is not detected in mutant embryos (E,G), except at the CP base (arrowheads) and in the hippocampal mantle (asterisks), as seen in controls (H). The tCPe of E16.5 mutants appears normal histologically (I). Broken lines outline the CP. Scale bars: 0.2 mm.