Fig. 3. hi^2499A mutants contain a mutagenic insertion in the vps18 gene. (A) Viral insertion in the vps18 gene at nucleotide 2236. Grey boxes indicate exons, horizontal lines indicate introns. Triangle represents virus. (B) cDNA prepared from day 5 phenotypically wild-type embryos and their mutant siblings from hi^2499A was amplified with vps18 and actin primers. (C) Unrooted phylogenetic tree for vps18. Bootstrap numbers are indicated. (D) Percent identity matrix for the Vps18 protein.