Fig. 5. Mutation of vps18 results in vesicle accumulation and lysosome depletion in hepatocytes. (A) hi^2499A mutants and vps18 morphants develop hepatomegaly. Scale bar: 100 µm. (B-D) Hematoxylin and Eosin staining of day 5 wild-type (B) and vps18 mutant (C,D) livers. Hepatocytes in mutant embryos are large and accumulate vacuoles (arrows in D). Boxed area in C is enlarged in D. Scale bar: 5 µm. (E,F) TEM of day 5 wild-type (E) and vps18 (F) hepatocytes shows large, membrane-bound structures proteinacious material and debris, indicative of vacuoles (v) in the mutants. n, nucleus. (G) vps18 mutation reduces the number of lysosomes in hepatocytes. Error bars indicate standard error of the mean.