Fig. 3. Analysis of the late-flowering fy and fca mutants. (A) Schematic diagram of FY gene and protein and position of fy mutations. The amino acid changes in fy-3 and fy-4 and nucleotide change in fy-1 are indicated in parentheses. Exons are represented as boxes and introns as lines. FY protein is represented as two protein domains, the WD repeats (7xWD) and C-terminal (CT) domain. (B) Phenotypes of late-flowering fy and fca mutations with (+VRN) and without (–VRN) 6-week vernalization treatments at 4°C. (B) The late-flowering mutants are grown alongside their wild-type, parental accession, either Ler (fy-1, fca-1) or Col (fy-2, fy-3, fca-9). (C) Northern blotting and hybridization analysis of FY, FLC and ß-TUB mRNA expression in wild-type, fy and fca mutant backgrounds. (D) Western blot analysis of FY accumulation in wild-type and fy mutant backgrounds. For northern and western blot analysis RNA and protein were extracted from non-vernalized, 10-day-old Arabidopsis seedlings.