Fig. 4. Cytoskeletal remodeling of dendrites during early metamorphosis. (A) Projected confocal z-stack of ddaC in the abdomen of a wild-type larva (ppk1.9GAL4> CD8::GFP). The box denotes the region from which images B-C' were collected. Scale bar: 30 µm. (B,B') The proximal branch of ddaC at wandering third instar larva stage labeled with anti-CD8 (B) and anti-Futsch (B'). Arrowhead indicates branches from another da neuron. (C,C') The proximal branch of ddaC at 3 h APF labeled with anti-CD8 (C), anti-Futsch (C'); arrows denote beads on branches. Filopodia (f) are found on branches that have undergone thinning; arrowhead indicates another da neuron. Scale bar: 10 µm. (D) Dynamics of the microtubule cytoskeleton of ddaC during the first six hours of metamorphosis. Projections of z-stacks from a movie showing tub::GFP distribution. 0 h APF reveals the uniform distribution of tub::GFP. By 2 h APF tubulin is redistributed resulting in a decrease in some places and an increase (arrow) in others. By 4 h APF some regions lose significant quantities of tub::GFP while others accumulate it into beads (arrow). By 6 h APF very large beads have formed that are separated by lengths of arbor that have little to no GFP signal. Scale bar: 30 µm.