Fig. 7. Phagocytic blood cells engulf neuronal debris and attack intact dendrites during pruning. (A) Frames from a time-lapse movie of da neurons (C161-GAL4>CD8::GFP) showing phagocytic blood cell consuming a detached dendrite branch. At 19 h 10 min a weakly labeled phagocyte enters from the right (arrowhead). By 19 h 40 min the phagocyte has moved close to the severed branch. At 19 h 50 min the branch fragments and by 20 h 50 min the phagocyte, is strongly labeled with GFP. Scale bar: 20 µm. (B) A projected z-stack of a phagocyte labeled with LysoTracker DND-99 in a wild-type animal (C161-GAL4>CD8::GFP) showing details of vesicles. Different sized vesicular compartments that do not contain GFP are evident (arrow) along with ones that do (arrowhead). Scale bar: 7 µm. (C) Phagocyte attacking distal tip of ddaE. Selected frames from a time-lapse movie. At 16 h 00 min a phagocyte can be seen on the right which then comes into contact with the distal tip of the branch (top arrowhead). By 17 h 00 min the branch and the phagocyte are no longer in contact and the phagocyte contains more GFP than before. Another phagocyte crosses the branch from the lower left (arrowhead) attacking the retracting end of the primary branch between 19 h 10 min and 20 h 30 min. Scale bar: 35 µm. (D) Phagocytic blood cells attack intact proximal branches. At 15 h 40 min a phagocyte enters the field from the left. Between 15 h 40 min and 17 h 30 min it moves close to the pruning arbor of ddaE (arrowhead). Between 18 h 10 min and 18 h 20 min the branch is severed at the site where the phagocyte is located. A small bleb of GFP, derived from the severed branch is internalized by the phagocyte and moves away with the blood cell (18 h 30 min arrowhead). Scale bar: 25 µm.