Fig. 8. Phagocyte behavior changes when pruning is blocked. False color indicates
the different frames of the respective time-lapse movies; magenta, the first
frame and green the final frame. The path of a phagocyte through all of the
frames is recorded as a white line, and the final destination denoted with an
arrow. (A) In a movie of a wild-type animal
(C161-GAL4>CD8::GFP) starting at 0 h (
14 h APF) the
phagocyte tracks around the ventral branch of ddaD until it is severed and
then moves to the right attacking the retracting branch of ddaE. (B) In a
movie of EcRDN expressing neurons
(C161-GAL4>EcRDN;CD8::GFP) starting at 0 h (14 h 20 min
APF) two phagocytes have been tracked. The phagocyte to the left follows the
distal tip of the branch, removing pieces as it retracts. The other phagocyte
is found near the proximal arbor and moves slowly across the field and does
not interact with the `non-thinned' branches. Scale bar: 30 µm.