Fig. 5. Elevated expression of miR166 in jba-1D plants. (A) Schematic of the insertion site of jba-1D relative to annotated genes. Green triangles indicate the 35S enhancers on the T-DNA vector. The orange line indicates the region of genomic DNA used to recapitulate the jba-1D phenotype. (B) Alignment of miR166 with its putative Arabidopsis class III HD-ZIP gene target sequences. The blue box indicates the miR166 sequence complementarity to the HD-ZIP genes sequences. (C) miR166 expression. Blot of low molecular mass RNA extracted from seedlings and inflorescences of wild-type and jba plants. The relative amount of miR166 accumulation in the various tissues is indicated below the autoradiograph. The ethidium bromide-stained gel is shown as loading control (bottom).