Fig. 2. Female meiosis is disrupted in rem mutants. (A,B,D,F) rem^HG24/rem^HG24, (C,E,G) wild type and (H) rem^RA74/Df(2L)30AC, labeled for DNA (green) and microtubules (red). (A) Metaphase I of meiosis is typically not affected in rem mutants, but 7% display disorganized spindles as in B. (C,D) Metaphase of meiosis II. In wild type, the meiosis II spindles are arrayed in tandem and connected by the spindle mid-body (arrow), while in rem, the spindles are kinked or separated. (E,F) Anaphase II. In wild type, four meiotic products with equal DNA content can be distinguished. rem spindles frequently break down and mis-segregation occurs. In the lower spindle, the two meiotic products are of different sizes, while the upper spindle has not entered anaphase. Pronuclear fusion in wild type (G) and rem (H). All nuclei are in interphase, and the male pronucleus and female pronucleus are in apposition and associated with the male sperm aster (arrows in G,H). The male pronucleus is largely obscured by the sperm aster in H. (I,J) Polar bodies from wild type (I) and rem^HG24/rem^RA74 (J) stained for microtubules (red) and the prophase/metaphase marker phospho-histone H3 (blue). Overlap appears pink. In wild type and in rem, DNA labels with phospho-histone H3, although in rem the chromatin is surrounded by microtubules within a shell, while in wild type the chromatin radiates out from a microtubule array. Scale bar: in A, 5 µm for A-H.