Fig. 1. Domain structure and expression patterns of Smed-netR in the intact and regenerating nervous system visualized by whole-mount in-situ hybridization. (A) Domain structure of Smed-netR compared to other members of the DCC family: frazzled (Drosophila), neogenin (mouse) and unc-40 (C. elegans). Smed-netR contains five predicted fibronectin type III repeats (NCBI CDD) (Marchler-Bauer and Bryant, 2004). (B,C) In intact planarians, Smed-netR is expressed in the cephalic ganglia, in the VNCs, in the nerve ganglia of the pharynx (asterisk) and in a few cells around the brain region. (D-I) Smed-netR expression during anterior regeneration. Arrowheads in E point to the brain primordia. (J-L) Smed-netR expression during posterior regeneration. Arrowheads in K point to the regenerated VNCs. Asterisks indicate the new pharynx. (B) Dark field image; (C-L) Nomarski differential interference contrast microscopy. All images are ventral views. (B-C) Anterior to the left. (D-L) Anterior to the top. Scale bars: 400 µm in B; 100 µm in C; 500 µm in D-I; 400 µm in J-L. cg, cephalic ganglia.