Fig. 3. Effects of Smed-netR RNAi upon uninjured regions of the animal and posterior regeneration. (A-B) Submuscular nerve plexus in the uninjured post-pharyngeal region of control (A) and dsRNA-injected (B) regenerating animals. The arrowheads point to some nerve fibers of the plexus. Asterisks mark the position of the VNCs deeper within the animal. (C-D) VNCs in the post-pharyngeal region of control (C) and dsRNA-injected (D) animals undergoing head regeneration. Ectopic nerve fibers appear between the VNCs of dsRNA-injected animals (arrowheads in D). (E-F) Newly formed VNCs in the regenerated tail 15 days after amputation; control (E) and dsRNA-injected (F) animals. Arrowheads in E and F point to the posterior end of the VNCs. (A) Confocal projection through 2 µm. (B) Confocal projection through 2.5 µm. (C,D) Single confocal planes. (E) Confocal projection through 2 µm. (F) Confocal projection through 2.5 µm. (A-C,E-F) Anterior to the left. (D) Anterior to the top. Scale bar: in F, 50 µm for A-D; 100 µm for E,F.