Fig. 3. Nasonia hunchback gene structure and the headless (hb^hl) deletion. (A) Nasonia hunchback gene structure [GenBank accession numbers: DQ116756 (cDNA), DQ116757 (cDNA), DQ116758 (genomic)]. Exons are boxed. Arrows indicate putative transcription start sites. ATG indicates putative initiating methionines. NF1 Zf, MF 1-4 Zf, and CF1-2 Zf refer to C2H2 zinc fingers, and are indicated as bars. The NF1 Zf is interrupted by an intron. The HB-GST region, against which the anti-Nv-Hunchback antibody was raised, is indicated as a stippled box in exon 4. TAA indicates the stop codon. The shaded box indicates a 3' UTR. (B) A deletion in Nasonia hunchback in the DNA from headless (hb^hl) mutant embryos. The open reading frames from headless and wild-type genomic DNA show that the breakpoints of the 1.497 kb hb^hl deletion generate a frameshift mutation in Nasonia hunchback (GenBank accession number: DQ116759). The dotted lines on the wild-type Nv hb indicate contiguous sequence. (C) The alignment of candidate NREs from the 3' UTR of Nasonia hunchback (Nvhb.1-4) with NREs of D. melanogaster (Dm), and with candidate NREs from hunchback genes of other insects: D. virilis (Dv), Tribolium (Tc), Locusta (Lm) and Schistocerca (Sa), as well as with Dm.cycB1.1.