Fig. 3. Epibranchial defects in mutants that disrupt endoderm. Lateral views, anterior to the left. (A,B) Wild type and cas mutant at 48 hpf, immunolabeled with anti-zn8 antibody, which labels pharyngeal pouches 1-6. (C,D) Wild type and cas mutant at 48 hpf, immunolabeled with anti-Hu to mark sensory neurons. (E,F) Wild type and cas mutant at 48 hpf labeled by whole-mount in situ hybridization for phox2b mRNA in neural progenitors. (G,H) Examples of phox2b expression in vgo mutants. Arrow in G denotes a ventrally extended ganglion; arrows in H denote fused or missing ganglia. (I,J) her5:egfp expression in living wild type and vgo mutant at 24 hpf. Arrows in I denote contacts between pouches and ectoderm; arrows in J denote deformed or missing pouches. 1-5, pharyngeal pouches; gM, medial portion of vagal; gP, posterior lateral line; gV, trigeminal; gIX, glossopharyngeal; gX, vagal; hb, hindbrain; mb, midbrain.