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Fig. 5. BMP gene expression in the pharyngeal pouches during epibranchial development, and defects in cas mutants. Lateral views, anterior to the left. Whole-mount in situ hybridization was performed in wild types at 22 hpf (top row), 30 hpf (second row) and 40 hpf (third row), and in cas mutants at 40 hpf (bottom row), for bmp2a (A,E,I,M), bmp2b (B,F,J,N), bmp4 (C,G,K,O) and bmp5 (D,H,L,P). Arrows indicate initial expression at the ventrolateral edges of each pouch. Arrow in H denotes bmp5 expression in the dorsal pouches. 1-5, pharyngeal pouches; ot, otic capsule.