Fig. 3. Effects of Khc mutations on endosome motion in oocytes during stages 10B-11. (A-D and Movies 4-6 in the supplementary material) Fluorescent yolk endosomes were imaged in isolated egg chambers during fast streaming stages. Each panel is a projection of 10 images from one focal plane acquired over 150 seconds. Fast-moving endosomes appear as strings of dots, while slower endosomes appear as blurred streaks. Non-moving endosomes appear as circular spots. Genotypes are indicated in each panel. Arrows in B,C indicate areas that had streaming movements. In C, the radial streaks at the upper right corner are from movement of debris outside the egg chamber. (E) Overall mean and peak endosome velocities are shown for each genotype. Numbers in or above bars show sample sizes for overall means. Peak velocities were calculated as means of the fastest 10% of endosomes for each genotype. Scale bar: 25 µm.