Fig. 2. Novel markers of transdetermining cells in Wg-expressing leg discs. (A) Schematic representation of a leg disc and the cuts performed to isolate transdetermining (TD, green), dorsal (D, D_WT, D_Wg, orange) and ventral cells (V, V_WT, V_Wg, blue). The area of the weak point is indicated by the dark-green circle. (B,C,D,F,G) In situ hybridization in wg-overexpressing leg discs. CG14059 (B), CG4914 (C) and CG12534 (D) are expressed in the area of the weak point. (E) A cultivated 3/4 fragment of a wild-type 1st leg disc. In the area of the weak point, Vg (E) and Ap protein (E') are both expressed in the dorsal region (E*, merged image: Vg, green; Ap, red) that is similar to the expression domain of CG14059; the expression domain of CG12534 is broader. Expression of lim1 RNA in normal (F) and a Wg-expressing leg disc (G). The distal expression domain of lim1 expands in the Wg-expressing disc (arrowheads) but is absent from an adjacent proximodistal region (outlined). Wg expression causes overgrowth of the dorsal region.