Fig. 1. Acinar-to-ductal metaplasia in primary explant cultures of mouse pancreas. (A,B) Phase contrast images of freshly harvested (day 0) non-transgenic pancreatic explant. (C) Hematoxylin and Eosin-stained section of non-transgenic day 0 explant. (D) Untreated non-transgenic day 5 explant. (E) Untreated MT-TGF day 5 explant cultured in the absence of exogenous growth factors. (F) Hematoxylin and Eosin-stained section of expanded duct-like epithelium arising from day 5 MT-TGF pancreas. (G) Non-transgenic day 5 explant cultured with 50 ng/ml rhTGF. (H) Non-transgenic day 5 explant cultured with 50 ng/ml rhHGF. (I) Non-transgenic day 14 explant cultured with 50 ng/ml rhTGF. (J) Quantification of duct-like structures formed following culture for 5 days with variable concentrations of either rhHGF (dark bars) or rhTGF (light bars). (K) Effect of EGF receptor-specific tyrosine kinase inhibitor EKI-785 on expansion of duct-like epithelium by either 50 ng/ml rhHGF (dark bars) or 50 ng/ml rhTGF (light bars). Values indicate mean±s.e.m. for three separate experiments; – – indicates no ductal structures observed.