Fig. 2. Acinar cell-specific recombination of the R26R reporter allele in Villin-Cre pancreas. (A) Low magnification view of neonatal pancreas from a Villin-Cre; R26R pup stained for ß-gal activity (blue) and counterstained with Eosin. (B) High-magnification view showing lack of ß-gal activity in islet and large duct. (C) High-magnification view showing an intralobular duct with no ß-gal activity and all cells with a clear acinar morphology positive for ß-gal. (D) High magnification view showing no ß-gal staining in terminal intercalated ducts that are most closely associated with acini. ß-Gal activity is lacking in the large blood vessel in the upper right corner. In all panels, ß-gal activity is present in all acinar cells. Scale bars: 10 µm. Arrows indicate pancreatic ducts. a, acinus; i, islet; v, blood vessel.