Fig. 10. Rescue of brain vascular abnormalities in embryos with the nestin-Psen1 transgene on the Psen1^–/– background. (A-C) Staining with isolectin B4 was performed on paraffin-embedded E12.5 embryos from wild-type (A) and Psen1^–/– (B) embryos, and embryos with the nestin-Psen1 transgene on the Psen1^–/– background (C). Sections through the lateral telencephalon are shown. Vessels in the Psen1^–/– embryo are enlarged. (D-L) Isolectin staining (E,H,K) was combined with nestin immunostaining (D,G,J) on paraffin-embedded E18.5 embryos from wild-type (D-F) and Psen1^–/– (G-I) embryos, and embryos with the nestin-Psen1 transgene on the Psen1^–/– background (J-L). Merged images are shown in F,I,L. Regions along the cortical surface of the lateral telencephalon are illustrated. The lectin-stained vessels in the Psen1^–/– brain are distorted and lobular appearance, and this pattern is normalized by the nestin-Psen1 transgene. The pattern of nestin staining is altered in the Psen1^–/– embryo. Scale bar: 100 µm for A-C; 50 µm for D-L.