Fig. 5. ß-Catenin regulates paraxis expression. (A) Schematic of the experiment: embryos were halved with one half processed for Fz7 expression and the other half processed for paraxis expression, as seen in D. (B) Schematic of the procedure: impermeable membranes were positioned at each side of the embryo and the embryos were allowed to grow for 10 hours. Embryos were then cut in half and processed for Fz7 and paraxis as before (E). (C) Somites (dorsal view) electroporated with a DN ß-catenin construct lose paraxis expression in the electroporated region (black arrowheads), whereas control somites express paraxis in the entire dermomyotome (white arrowheads). (F,G) Dorsal electroporation of DN Lef1 construct. Electroporated cells, which express GFP (G), do not express paraxis (F).