Fig. 6. EGFR phosphorylation in recombined mammary glands. Tissue extracts were immunoprecipitated (IP) with anti-EGFR antibodies, immunoblotted (IB) for phosphotyrosine (pTyr) or phosphorylated EGFR (pEGFR; Y1068), and re-probed for EGFR. Western blots (WB) for keratin 14 were run on the original extracts and re-probed for ß-actin. Renal transplants with the indicated epithelial and stromal genotypes (wt, wild type; 17^–/–, Adam17^–/–; A^–/–, Areg^–/–; R^–/–, Egfr^–/–) were harvested 2 weeks after transplantation without exogenous estradiol. Control tissues were harvested from 6-week-old wild-type male (M) and female (F) mice, and Egfr^–/– neonates (R^–/– p0) 15 minutes after intraperitoneal injection of EGF (2.5 mg/kg body weight) or phosphate-buffered saline (PBS).