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Fig. 2. JNK signaling promotes the loss of puc mutant imaginal tissues. (A-D) Third-instar wing discs. GFP-expressing (green) cells are clonal patches of cells homozygous for: (A) wild-type control, (B) pucA251.1, (C) pucR10, (D) UAS-myc-Puc; pucA251.1. Actin (purple). puc mutant clones are not recovered unless Puc+ function is trangenically restored (D). The GFP-signal in B is from clones outside the disc proper in the parapodial membrane. (E-I) Adult eyes. All eye cells homozygous for: (E) wild-type control, (F) pucA251.1, (G) pucR10, (H) bsk2/+; pucA251.1 and (I) pucA251.1 p53[5A-1-4]. Eye tissue reappears in bsk heterozygotes or p53 homozygotes.