Fig. 1. Distribution patterns of PhGFP and PcGFP in the embryos and larval wing discs. (A-C) Western blot analysis of Drosophila tissue extracts from wild-type and transgenic fly lines. (A) Extracts from wing discs from wild-type and PhGFP-expressing larvae (probed against Polyhomeotic). (B) Extracts from wild-type and PcGFP-expressing embryos (probed against Polycomb). (C) Extracts from salivary glands from PcGFP- and PhGFP-expressing larvae (probed against GFP). Antibodies against Poly-A-binding protein (PABP) (A), S6 Ribosomal protein (B) and eIF4A (C) were used for loading control. PhGFP expression was induced using the en:GAL4 driver in embryos (D) and the BX^MS1096:Gal4 driver in wing imaginal discs (H). The corresponding distribution pattern of PhGFP in nuclei can be seen in E (embryos) and I (wing discs). PcGFP expression in embryos and wing imaginal discs is shown in F and J, and the respective nuclei in G and K. Scale bars: 5 µm.