Fig. 2. Diffusion of PcGFP in nuclei of preblastoderm embryos. A square-shaped region (1.5x1.5 µm) in the center of a preblastoderm nucleus (A) of a PcGFP embryo was photobleached and the fluorescence recovery was measured over time. (B) Data points for 40 FRAP curves from similar nuclei as in (A) were averaged and fitted to a hyperbolic function (Eqn 2 in the Materials and methods). Scale bar: 5 µm.