Fig. 5. Dissociation rate constants for PhGFP and PcGFP. Individual PcG protein loci in the diploid nuclei of the embryos and wing imaginal discs were subjected to 3D-iFRAP. Fluorescence decay curves were fitted to a single exponential function. (A) Histogram of dissociation rate constants of individual PhGFP loci obtained from 3D-iFRAP experiments in embryos and larval wing imaginal discs are shown in red and black, respectively. (B) A single dissociation rate constant could be fitted by averaging all 30 normalized data from PhGFP embryos (k_off=0.051±0.004 second^–1). (C) Average of all 32 normalized data from PhGFP wing discs (k_off=0.032±0.002 second^–1). (D) Average dissociation rate curve for data from 10 determinations of PcGFP loci in imaginal discs (k_off=0.034±0.003 second^–1).