Fig. 6. CyA treatment disrupts ANC formation. Flat-mounted, Alcian Blue stained cartilages at 5 dpf; anterior is to the left. (A) Wild type. (B) syu mutant. (C) A wild-type treated with 5 µM CyA from 4-8 hpf. (D-I) Wild-type larvae treated with 50 µM CyA at different stages between 18-60 hpf. (D,E) Treatment between 4-24 hpf eliminates all ANC cartilage. (F) Treatment between 24-30 hpf eliminates most cartilage, although some trabecular cartilage forms but never fuses to the posterior neurocranium. (G) Treatments between 30-36 hpf eliminate the ethmoid, but not trabeculae. (H,I) Treatments later than 40 hpf cause slight reductions in the ANC. abc, anterior basicapsular commissure; bp, basal plate; ep, ethmoid plate; nc, notochord; pbc, posterior basicapsular commissure; pc, parachordal cartilage; tc, trabeculae.