Fig. 5. Ultrastructural analysis of RNF17 granules by immuno-EM. Affinity purified anti-RNF17 antibodies (anti-serum 1774) were used as primary antibodies. Secondary antibodies were labeled with 10 nm gold particles. (A) The RNF17 granule in a spermatocyte. (B) The RNF17 granule in a spermatid. (C) The RNF17 granule in a cytoplasmic residual body. Arrows indicate the accumulation of gold particles in the 500 nm RNF17 granules. Immuno-EM without the primary antibody was negative (data not shown). cb, chromatoid body; m, mitochondria; n, nucleus; v, vacuole.