Fig. 7. Targeted disruption of the Rnf17 gene. (A) Schematic diagram of the wild-type allele, the targeting vector and the targeted allele. PGK-Neo: neomycin-resistance gene driven by the PGK1 promoter. PCR primers (P1, P2, P3 and P4) used for genotyping are indicated. (B) PCR analysis of genomic DNA from Rnf17 mice. +/+, wild type; +/-, heterozygote; -/-, homozygote. All four primers were included in each PCR assay. WT, wild-type allele; MT, mutant allele. (C) Absence of RNF17 proteins in the Rnf17-deficient testes. Western blot analysis was done with both anti-sera 1774 (as shown) and GP8 (data not shown).