Fig. 3. Increased numbers of myt1 mutant secondary spermatogonia. (A,D) BrdU-incorporation labels S-phase cells (green), showing that more cells are labeled in myt1 mutant testes (D) than in controls (A). (B,E) Fas3 labeling of hub cells (green, arrow) and Vasa germline-specific staining indicates the position of germline stem cells (outlined), showing that there are similar numbers of stem cells in myt1 mutants (E) and controls (B). (C,F) BamC (green) staining of secondary spermatogonia shows more spermatogonia in myt1 mutants (F) than in controls (C). Scale bars: 16 µm for A,C,D,F; 8 µm for B,E.