Fig. 6. EPHB4 decreases chemokine-stimulated cytotrophoblast migration. (A) RT-PCR detected expression of PDZ-RGS3 in RNA samples isolated from first trimester (1T) and second trimester (2T) cytotrophoblasts (CTB). PBMCs, peripheral blood mononuclear cells. +, positive control; –, negative control. (B) Cytokeratin 7 staining of cytotrophoblasts that had migrated to the underside of the transwell filter after overnight culture. EPHB4 decreased cytotrophoblast migration towards chemokines, while baseline and growth factor-stimulated migration was not significantly affected. (C) Quantification of migration assays showed that EPHB4 downregulated chemokine-induced migration by an average of 60%. Error bars indicate s.d.