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Fig. 1. T-DNA insertion mutations in ARF6 and ARF8. (A) Locations of T-DNA insertions. Horizontal bars represent ARF6 (At1g30330) and ARF8 (At5g37020) -coding regions, with exons (black) and introns (white) indicated. (B) RNA gel blot hybridization using ARF6 and rDNA probes. (Left panel) RNA from 8-day-old wild-type seedlings, arf6-2 mutant seedlings or wild-type flowers, stems, leaves or roots. (Right panel) RNA from wild-type or mutant flowers. (C) RNA gel blot hybridization using ARF8 and rDNA probes. Left panel, RNA from wild-type seedlings, arf8-3 mutant seedlings or wild-type flowers, stems, leaves or roots. (Right panel) RNA from wild-type or mutant flowers.