Fig. 5. Effects of arf6-2 and arf8-3 mutations on gene expression in flowers. (A) Numbers of genes with expression that differs by at least 2.5-fold between different stages in wild-type flowers, grouped by stage of highest expression, together with numbers of genes in each wild-type expression class that were also developmentally regulated in arf6-2/arf6-2 arf8-3/ARF8 sesquimutant and arf6-2 arf8-3 double mutant flowers. (B,C) Venn diagrams showing numbers of genes that were underexpressed (B) or overexpressed (C) at different stages in arf6-2 arf8-3 double mutant flowers, and in arf6-2/arf6-2 arf8-3/ARF8 sesquimutant flowers (in parentheses). (D) Gene expression in response to auxin and jasmonic acid. Wild-type or arf6-2 arf8-3 mutant mature flowering plants were mock-treated or treated with IAA or JA for the times indicated, and blots of RNA from inflorescence apices were hybridized with the indicated probes.