Fig. 6. mls-2 is specifically required for the M lineage expression of hlh-1. (A,B) A lin-39(n1760) mab-5(e1239) mutant animal (A) and a hlh-1(cc561ts) mutant animal (B) stained with anti-MLS-2 antibodies, showing mls-2 expression in M (arrow) and in six head neurons (arrowhead). (C) M lineage expression (arrow) of mab-5::gfp in a cc615 mutant animal. (D-F) A mls-2(cc615) ayIs6(hlh-8::gfp) mutant animal at the 4-M stage stained with anti-HLH-1 antibodies (red in D,E) and DAPI (F). Note that HLH-1 was present in the embryonic BWMs (white arrows in D-F), but not in the four M granddaughter cells (blue arrows in E-F).