Fig. 7. VE-cadherin and CD45 expression within the E11.5 and E12.5 placental CD34⁺KIT⁺ population. (A) CD34 and KIT expression profile of the E11.5 placenta. CD34⁺KIT⁺ were gated (black box) and analysed for VE-cadherin and CD45 expression. Contour plot is composed from 1x10⁵ cells. (B) All E11.5 CD34⁺KIT⁺ cells co-express VE-cadherin and CD45. (C) At E12.5, the placenta CD34⁺KIT⁺ population has markedly lost VE-cadherin expression. Analyses in B and C were made using data from from 2000-4000 gated cells. All data are representative of three experiments. Isotype control (white) and specific antibody staining (grey) are presented.