Fig. 2. Tcre;Fgfr1 skeletal phenotypes and gene expression. (A-H) Skeletal preparations of E17.5 limbs, stained for cartilage (Alcian Blue) and bone (Alizarin Red). (A-D) All segments of the limb, the stylopod (S), zeugopod (Z) and autopod (A) are reduced in the mutant, while the structures outside of the limb, the scapula (sc) and pelvic girdle (pg), remain normal. (E-H) Magnified views of autopod skeletons. The positions of metacarpals (mc) and metatarsals (mt) are indicated. Dots in F and H indicate individual phalange. (I-P) Forelimb buds at E11.5 (I,J) and E12.5 (K-P). Arrowheads in K-P indicate the position of the most anterior digit.