Fig. 4. Late-born matrix neurons develop normally in the absence of Notch1. Ebf1 expression and ephrin A4/Fc binding is enriched in the matrix compartment of the striatum, and excluded from the SCS and patch regions. The patterns of Ebf1 and ephrin A4/Fc staining appear to be equivalent in both wild-type embryos and Foxg1Cre; N1 cKOs, as seen here in coronal sections through the striatum at E18.5. The SCS, which can be visualized by the lack of Ebf1 and ephrin A4 staining (shown in green), is expanded in the absence of Notch1 (dashed lines). The patch compartments, which also exclude Ebf1 and ephrin A4, express Darpp32 (shown in red) and are reduced at E18.5 in the mutant (arrows). Scale bar: 200 µm.