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Fig. 6. BDNF prevents the effects that NMDA receptor blockade exerts on RGCs. Reconstructions of three-dimensional arbors illustrate the effects of APV and BDNF treatments on axon arbor complexity and GFP-synaptobrevin cluster number. Individual RGC axons double-labeled with GFP-synaptobrevin and DsRed2 were visualized by confocal microscopy in the live developing tadpole after tectal injection of (A) control vehicle solution, (B) APV or (C) APV plus BDNF. (B) A significant decrease in the number and density of GFP-synaptobrevin clusters in RGC axon arbors is observed 2 hours after APV treatment. GFP-synaptobrevin cluster density and arbor complexity begin to recover by 24 hours and further develop 9 days after treatment. (C) BDNF maintained GFP-synaptobrevin cluster density for most of the observation period in RGC axon arbors treated with APV. Posterior is upwards, anterior is downwards. Scale bar: 20 µm.