Fig. 6. Loss of cut function during early oogenesis is sufficient to drive the follicle cells to differentiate. ct mutant cells are marked by the absence of GFP (green in A,B,E,F). FasIII staining is shown in red (A-D) and white (A'-D'). DAPI is shown in blue (A-E). (A,B) FasIII expression was downregulated and was restricted to the apical-lateral regions (arrowheads in B and B') in ct^db7 clones (outlined) in early stages. (C,D) Different FasIII expression patterns before and after stage 7 in the wild type. During stage 8 (S8), FasIII expression in the basal lateral region was downregulated, whereas the apical lateral region retained FasIII expression (C,C'). In contrast, FasIII was expressed in all lateral regions of the follicle cells at stage 6 (D,D'). The apical (Ap) and basal (Ba) surfaces of the follicle cells are outlined (C',D'). (E,F) Eya (red in E, blue in F, and white in E',F') was also downregulated in ct^db7 follicle-cell clones (outlined) in early stages, whereas wild-type follicle cells, except the polar cells (arrow in F), expressed Eya. ct^db7 follicle-cell clones did not express the polar cell marker, PZ80-lacZ (red in F).