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Fig. 3. Nodal and Pitx2 expression in wild-type and Sufu-/- mouse embryos. (A,B) Whole-mount RNA in situ using a digoxigenin-labeled Nodal riboprobe on 7.5 dpc embryos shows Nodal expression in the region of the node in a wild-type embryo (A) and marked expansion of the region of Nodal expression in a Sufu-/- embryo (B). (C,D) Whole-mount in situ hybridization using a Pitx2 digoxigenin-labeled riboprobe on 8.5 dpc embryos (posterior views) shows Pitx2 expression in the head folds and left lateral plate mesoderm in a wild-type embryo (C); Pitx2 expression is absent in the left lateral plate mesoderm of a Sufu-/- embryo (D).