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Fig. 5. Derepression of Shh RNA expression in Sufu-/- mutants. Whole-mount in situ hybridization using a Shh digoxigenin-labeled riboprobe on wild-type (A) and Sufu-/- (B) embryos at 9.5 dpc. Sufu-/- (B) embryos exhibited a dramatic upregulation of Shh expression throughout the brain and developing spinal cord when compared with wild type. Cross-section of a wild-type embryo (C) demonstrates that Shh is normally expressed in the floor plate; the Sufu-/- embryo (D) displays a dorsal expansion of Shh expression throughout the neural tube. Cross-sections are of the thoracic region. Scale bars: 100 µm.