Fig. 7. Pax7 expression in wild type and Sufu^-/- mutants. Whole-mount in situ hybridization using a Pax7 digoxigenin-labeled riboprobe on wild-type (A) and Sufu^-/- (B) embryos at 9.5 dpc. The Sufu^-/- embryo exhibited virtually no Pax7 expression throughout the brain and developing spinal cord compared with wild type. Cross-section of a wild-type embryo (C) demonstrates that Pax7 is normally expressed in the dorsal neural tube; a Sufu^-/- embryo (D) displays virtually no expression throughout the neural tube. Cross-sections are of the thoracic region.