Fig. 2. Aggregating neural crest cells as they form the DRG anlagen. Embryos were injected at HH stage 10 with GFP coding vector pMES and sagittal explant mounted at embryonic day 3.5. (A) Neural crest cells migrating from three axial levels through the rostral somite, with arrowhead indicating cells between two DRG anlagen. Double-ended arrow indicates dorsal (D) and ventral (V) directions. (B) High magnification of area indicated by arrowhead in (A). Cells from DRG1 and DRG2 are in contact with each other. (C-D) Cell tracking of a single neural crest cell that initially resided in one DRG and then migrated to the DRG immediately posterior. Scale bars: 20 µm in A; 40 µm in C-E.