Fig. 3. Positional cloning of esrom. (A) Map of zebrafish chromosome 9,
depicting the region to which esrom maps and its synteny to human
chromosome 13 (orange). Yellow color indicates synteny to human chromosome 2.
(B) Genomic contig assembly of zebrafish PAC and BAC clones encompassing
esrom and its similarity to human chromosome 13q22.3. esr
maps 0.5 cM from z6663, 0.2 cM from fj33d03 and less than 0.08 cM from
fa97c06. (C,D) Retinotectal projections of DiI-labeled anterior RGC axons in
4-day-old wild-type embryos injected with control morpholino and zebrafish PAM
morpholino. (C) In the control, anterior RGC axons branch in the posterior
tectum as in wild types. (D) In zebrafish, PAM morphants anterior RGC axons
branch prematurely in the anterior tectum (arrow). (E,F) Lateral view of
3-day-old larvae. Xanthophore phenotype in morphants mimics that of mutants
(Le Guyader et al., 2004). (G)
Splice site target of zebrafish PAM morpholino2. (H) RT-PCR of 400 bp
unspliced transcript in control and morphants. The unspliced band is amplified
only in the zebrafish PAM morphants. (I) Single cDNA band (13 kb) from RT-PCR
using esrom specific primers. (J) Point mutation in
esrtp03. Scale bar: 25 µm.