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Fig. 4. Altered expression of matrix-degrading metalloproteinases and their inhibitors in K5-{Delta}N57-ßcat mouse mammary glands. Semi-quantitative RT-PCR analysis of Mmp and Timp transcript levels in wild-type (WT) and K5-{Delta}N57-ßcat (K5-{Delta}Nß) mouse mammary glands. 18S RNA was used as a normalization control for RT-PCR. Samples from wild-type and K5-{Delta}N57-ßcat glands were analyzed in the same gel. P7.5, P10.5 and P13.5 correspond to 7.5, 10.5 and 13.5 days of pregnancy; L2 and L6, to 2 and 6 days of lactation; I3, to 3 days of involution.