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Fig. 6. Basal-type mammary hyperplasia in 12- to 18-month-old K5-{Delta}N57-ßcat mice. (A) Whole-mount staining of 18-month-old wild-type (WT) and K5-{Delta}N57-ßcat nulliparous mouse mammary gland fragments containing large ducts. (B) Sections through two 18-month-old K5-{Delta}N57-ßcat nulliparous mouse mammary glands stained with anti-K5 antibody. Note focal thickenings of ductal epithelium (arrows, upper panel) and large hyperplastic areas (lower panel) containing K5-positive cells. (C) Double indirect immunofluorescence staining of a section through a 16-month-old nulliparous. K5-{Delta}N57-ßcat mouse mammary gland containing focal epithelial thickenings with anti-K5 and anti-HA-tag-antibodies revealing transgene product. There are numerous HA-positive nuclei (arrows). (D,E) Serial sections through a wild-type (WT) and a 16-month-old K5-{Delta}N57-ßcat nulliparous mouse mammary gland containing focal epithelial thickenings double stained with anti-K5 and anti-K8 (D) or with anti-K5 and anti-{alpha}-SM-actin-antibodies (E). Cells in the hyperplastic areas are K5 positive, but do not express K8 or {alpha}-SM-actin. The broken line indicates the position of the basement membrane. (F) Double indirect immunofluorescence staining of a section through a hyperplastic area from a 12-month-old multiparous K5-{Delta}N57-ßcat mouse mammary gland with anti-laminin and anti-p63 antibodies. The staining of the basement membrane with the anti-laminin antibody is discontinuous around hyperplastic ducts filled with p63-expressing cells (arrows). Scale bars: 0.6 mm in A; 0.2 mm in B; 30 µm in C-E; 50 µm in F.