Fig. 8. Levels of Myc, cyclin D1, Timp3 and p63 transcripts in the mammary hyperplasia in K5-N57-ßcat mice. (A) Real-time RT-PCR analysis of levels of Myc, cyclin D1 and Timp3 transcripts in hyperplastic and tumor-free tissue samples from 12- to 18-month-old transgenic glands (K5-Nß). Bars represent the mean values x10^-2±s.e.m. obtained in two independent experiments performed in duplicate. (B) Real-time RT-PCR analysis of TAp63 and Np63 transcript levels in wild-type and transgenic glands. Bars represent the mean values x10^-4±s.e.m. obtained for RNA samples isolated from hyperplastic and tumor-free areas from four 12- to 18-month-old K5-N57-ßcat (K5-Nß) and four wild-type (WT) mice. The differences in Np63 transcript levels were statistically significant between wild-type and transgenic tumor-free specimens, wild-type and transgenic hyperplastic tissue, and transgenic tumor-free and transgenic hyperplastic tissue (P<0.003, 0.001 and 0.002, respectively). The differences in TAp63 transcript levels were statistically significant between wild-type and transgenic tumor-free specimens, and between wild-type and transgenic hyperplastic tissue (P<0.0015 and 0.002, respectively). Transcript levels were normalized to the Gapd mRNA.