Fig. 7. Localization of a GL3-YFP translation fusion in the root epidermis. Expression of a GL3::GL3-YFP construct in the developing root epidermis of 4-day-old gl3-2 mutant seedlings. First three panels: wide-field fluorescence microscope images. Stars indicate cells files composed of developing non-hair cells, as determined by tracing the files to the mature region of the root. Right-most panel: confocal microscope image (cell walls were counterstained with propidium iodide (red signal) to enhance visualization). The GL3 fusion protein accumulates predominantly in the nuclei of the developing non-hair cells, as apparent from their lower cell division rate and paired nature of these cell files. Consistent with the GL3::GUS expression pattern (Fig. 1), the GL3 fusion protein also accumulates in the quiescent center cells of the root meristem.